Search for differentially expressed proteins involved in the treatment of human nasopharyngeal carcinoma cells with NGX6 using two-dimensional electrophoresis and mass spectrometry

Jiang Li, Chen Tan, Qiu Xiang, Xiao Mei Zhang, Jian Ma, Wei Fang Li, Jie Ru Wang, Jian Bo Yang, Song Ping Liang, Gui Yuan Li

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The current study was designed to reveal the preliminary function of NGX6 to NPC cells. In search of mechanisms of NGX6 2-D PAGE was used to identify proteins that were overexpressed in HNE cells that were transfected with NGX6. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry. Seven proteins such as Fas, ZNF and MHC-II Ag were identified. The possible significance of these findings is discussed. NGX6 may exert its effect on NPC cells by many ways.

Original languageEnglish (US)
Pages (from-to)573-577
Number of pages5
JournalProgress in Biochemistry and Biophysics
Volume28
Issue number3
StatePublished - 2001

Keywords

  • Cell transfection
  • Ionization mass spectrometry
  • Matrix-assisted laser desorption
  • Two-dimensional gel electrophoresis

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