Search for differentially expressed proteins involved in the treatment of human nasopharyngeal carcinoma cells with NGX6 using two-dimensional electrophoresis and mass spectrometry

Jiang Li; Chen Tan; Qiu Xiang; Xiao Mei Zhang; Jian Ma; Wei Fang Li; Jie Ru Wang; Jian Bo Yang; Song Ping Liang; Gui Yuan Li

Search for differentially expressed proteins involved in the treatment of human nasopharyngeal carcinoma cells with NGX6 using two-dimensional electrophoresis and mass spectrometry

Jiang Li, Chen Tan, Qiu Xiang, Xiao Mei Zhang, Jian Ma, Wei Fang Li, Jie Ru Wang, Jian Bo Yang, Song Ping Liang, Gui Yuan Li

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The current study was designed to reveal the preliminary function of NGX6 to NPC cells. In search of mechanisms of NGX6 2-D PAGE was used to identify proteins that were overexpressed in HNE cells that were transfected with NGX6. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry. Seven proteins such as Fas, ZNF and MHC-II Ag were identified. The possible significance of these findings is discussed. NGX6 may exert its effect on NPC cells by many ways.

Original language	English (US)
Pages (from-to)	573-577
Number of pages	5
Journal	Progress in Biochemistry and Biophysics
Volume	28
Issue number	3
State	Published - 2001

Keywords

Cell transfection
Ionization mass spectrometry
Matrix-assisted laser desorption
Two-dimensional gel electrophoresis

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Search for differentially expressed proteins involved in the treatment of human nasopharyngeal carcinoma cells with NGX6 using two-dimensional electrophoresis and mass spectrometry. / Li, Jiang; Tan, Chen; Xiang, Qiu et al.
In: Progress in Biochemistry and Biophysics, Vol. 28, No. 3, 2001, p. 573-577.

Research output: Contribution to journal › Article › peer-review

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abstract = "The current study was designed to reveal the preliminary function of NGX6 to NPC cells. In search of mechanisms of NGX6 2-D PAGE was used to identify proteins that were overexpressed in HNE cells that were transfected with NGX6. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry. Seven proteins such as Fas, ZNF and MHC-II Ag were identified. The possible significance of these findings is discussed. NGX6 may exert its effect on NPC cells by many ways.",

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AU - Tan, Chen

AU - Xiang, Qiu

AU - Zhang, Xiao Mei

AU - Ma, Jian

AU - Li, Wei Fang

AU - Wang, Jie Ru

AU - Yang, Jian Bo

AU - Liang, Song Ping

AU - Li, Gui Yuan

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KW - Cell transfection

KW - Ionization mass spectrometry

KW - Matrix-assisted laser desorption

KW - Two-dimensional gel electrophoresis

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Search for differentially expressed proteins involved in the treatment of human nasopharyngeal carcinoma cells with NGX6 using two-dimensional electrophoresis and mass spectrometry

Abstract

Keywords

UN SDGs

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