TY - JOUR
T1 - Short communication
T2 - HIV type 1 escapes inactivation by saliva via rapid escape into oral epithelial cells
AU - Dietrich, Elizabeth A.
AU - Gebhard, Kristin H.
AU - Fasching, Claudine E.
AU - Giacaman, Rodrigo A.
AU - Kappes, John C.
AU - Ross, Karen F
AU - Herzberg, Mark C
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Saliva contains anti-HIV-1 factors, which show unclear efficacy in thwarting mucosal infection. When incubated in fresh, unfractionated whole saliva, infectious HIV-1 IIIb and BaL (X4- and R5-tropic, respectively) persisted from 4 to at least 30min in a saliva concentration-dependent manner. In salivary supernatant for up to 6h, both infectious HIV-1 strains "escaped" into immortalized oral epithelial cells; infectious BaL showed selectively enhanced escape in the presence of saliva. Fluorescently labeled HIV-1 virus-like particles entered oral epithelial cells within minutes of exposure. Using a previously unrecognized mechanism, therefore, strains of HIV-1 escape inactivation by saliva via rapid uptake into oral epithelial cells.
AB - Saliva contains anti-HIV-1 factors, which show unclear efficacy in thwarting mucosal infection. When incubated in fresh, unfractionated whole saliva, infectious HIV-1 IIIb and BaL (X4- and R5-tropic, respectively) persisted from 4 to at least 30min in a saliva concentration-dependent manner. In salivary supernatant for up to 6h, both infectious HIV-1 strains "escaped" into immortalized oral epithelial cells; infectious BaL showed selectively enhanced escape in the presence of saliva. Fluorescently labeled HIV-1 virus-like particles entered oral epithelial cells within minutes of exposure. Using a previously unrecognized mechanism, therefore, strains of HIV-1 escape inactivation by saliva via rapid uptake into oral epithelial cells.
UR - http://www.scopus.com/inward/record.url?scp=84870017035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870017035&partnerID=8YFLogxK
U2 - 10.1089/aid.2011.0069
DO - 10.1089/aid.2011.0069
M3 - Article
C2 - 22077822
AN - SCOPUS:84870017035
SN - 0889-2229
VL - 28
SP - 1574
EP - 1578
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 12
ER -
