Simultaneous detection and deconvolution of congested NMR spectra containing three isotopically labeled species

Larry R. Masterson, Marco Tonelli, John L. Markley, Gianluigi Veglia

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We present a procedure for isolating subspectra corresponding to individual protein or peptide components in a ternary mixture or complex. Each of the three-component species is labeled differently: species A uniformly with 15N, species B uniformly with 15N and 13C, and species C uniformly with 15N but selectively with 13C′ or 13Cα. By using the dual carbon label selective HSQC (DCLS-HSQC) pulse sequence and exploiting differences in 1J 15N-13C coupling patterns to filter selected 15N resonances from detection during a constant time period, a subspectrum from each species can be generated from three spectra acquired from a single sample. Many important biological pathways involve dynamic interactions among members of multicomponent protein assemblies, and this approach offers a powerful way to monitor such processes.

Original languageEnglish (US)
Pages (from-to)7818-7819
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number25
DOIs
StatePublished - Jun 25 2008

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