TY - JOUR
T1 - Single-Molecule Dual-Beam Optical Trap Analysis of Protein Structure and Function
AU - Sung, Jongmin
AU - Sivaramakrishnan, Sivaraj
AU - Dunn, Alexander R.
AU - Spudich, James A.
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.
AB - Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.
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U2 - 10.1016/S0076-6879(10)75014-X
DO - 10.1016/S0076-6879(10)75014-X
M3 - Article
C2 - 20627164
AN - SCOPUS:77954599241
SN - 0076-6879
VL - 475
SP - 321
EP - 375
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -