Abstract
The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1- and SSEA-1+ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 101-104 |
| Number of pages | 4 |
| Journal | Journal of veterinary science |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2017 |
Bibliographical note
Funding Information:We thank Drs. J. Ohlfest, E. Pluhar, C. Bird, B. Rütgen, S. Helfand, and E. Dickerson for providing cell lines. This study was supported by the grants from the NIH Comprehensive Cancer Center Support Grant to the Masonic Cancer Center (P30 CA077598) in United States and the Fujian Fuming Foundation in China.
Publisher Copyright:
© 2017 The Korean Society of Veterinary Science. All Rights Reserved.
Keywords
- Canine cancer
- Flow cytometry
- Magnetic-activated cell sorting
- Stage-specific embryonic antigens
