Abstract
Magnetic resonance spectroscopybased magnetization transfer techniques (MT) are commonly used to assess the rate of oxidative (i.e., mitochondrial) ATP synthesis in intact tissues. Physiologically appropriate interpretation of MT rate data depends on accurate appraisal of the biochemical events that contribute to a specific MT rate measurement. The relative contributions of the specific enzymatic reactions that can contribute to a MT Pi¡ATP rate measurement are tissue dependent; nonrecognition of this fact can bias the interpretation of MT Pi¡ATP rate data. The complexities of MT-based measurements of mitochondrial ATP synthesis rates made in striated muscle and other tissues are reviewed, following which, the adverse impacts of erroneous Pi¡ATP rate data analyses on the physiological inferences presented in selected published studies of cardiac and skeletal muscle are considered.
Original language | English (US) |
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Pages (from-to) | C1-C11 |
Journal | American Journal of Physiology - Cell Physiology |
Volume | 301 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2011 |
Keywords
- Magnetization transfer
- Oxidative ATP synthesis
- Striated muscle