TY - JOUR
T1 - Strain level streptococcus colonization patterns during the first year of life
AU - Wright, Meredith S.
AU - McCorrison, Jamison
AU - Gomez, Andres M.
AU - Beck, Erin
AU - Harkins, Derek
AU - Shankar, Jyoti
AU - Mounaud, Stephanie
AU - Segubre-Mercado, Edelwisa
AU - Mojica, Aileen May R.
AU - Bacay, Brian
AU - Nzenze, Susan A.
AU - Kimaro, Sheila Z.M.
AU - Adrian, Peter
AU - Klugman, Keith P.
AU - Lucero, Marilla G.
AU - Nelson, Karen E.
AU - Madhi, Shabir
AU - Sutton, Granger G.
AU - Nierman, William C.
AU - Losada, Liliana
N1 - Funding Information:
This work was supported by grant OPP1017579 from the Bill and Melinda Gates Foundation.
Publisher Copyright:
© Wright, McCorrison, Gomez, Beck, Harkins, Shankar, Mounaud, Segubre-Mercado, Mojica, Bacay, Nzenze, Kimaro, Adrian, Klugman, Lucero, Nelson, Madhi, Sutton, Nierman and Losada.
PY - 2017/9/6
Y1 - 2017/9/6
N2 - Pneumococcal pneumonia has decreased significantly since the implementation of the pneumococcal conjugate vaccine (PCV), nevertheless, in many developing countries pneumonia mortality in infants remains high. We have undertaken a study of the nasopharyngeal (NP)microbiome during the first year of life in infants fromThe Philippines and South Africa. The study entailed the determination of the Streptococcus sp. carriage using a lytA qPCR assay, whole metagenomic sequencing, and in silico serotyping of Streptococcus pneumoniae, as well as 16S rRNA amplicon based community profiling. The lytA carriage in both populations increased with infant age and lytA+ samples ranged from 24 to 85% of the samples at each sampling time point. We next developed informatic tools for determining Streptococcus community composition and pneumococcal serotype from metagenomic sequences derived from a subset of longitudinal lytA-positive Streptococcus enrichment cultures fromThe Philippines (n = 26 infants, 50% vaccinated) and South African (n = 7 infants, 100% vaccinated). NP samples from infants were passaged in enrichment media, and metagenomic DNA was purified and sequenced. In silico capsular serotyping of these 51 metagenomic assemblies assigned known serotypes in 28 samples, and the co-occurrence of serotypes in 5 samples. Eighteen samples were not typeable using known serotypes but did encode for capsule biosynthetic cluster genes similar to non-encapsulated reference sequences. In addition, we performed metagenomic assembly and 16S rRNA amplicon profiling to understand co-colonization dynamics of Streptococcus sp. and other NP genera, revealing the presence of multiple Streptococcus species as well as potential respiratory pathogens in healthy infants. A range of virulence and drug resistant elements were identified as circulating in the NP microbiomes of these infants. This study revealed the frequent co-occurrence of multiple S. pneumoniae strains along with Streptococcus sp. and other potential pathogens such as S. aureus in the NP microbiome of these infants. In addition, the in silico serotype analysis proved powerful in determining the serotypes in S. pneumoniae carriage, and may lead to developing better targeted vaccines to prevent invasive pneumococcal disease (IPD) in these countries. These findings suggest that NP colonization by S. pneumoniae during the first years of life is a dynamic process involving multiple serotypes and species.
AB - Pneumococcal pneumonia has decreased significantly since the implementation of the pneumococcal conjugate vaccine (PCV), nevertheless, in many developing countries pneumonia mortality in infants remains high. We have undertaken a study of the nasopharyngeal (NP)microbiome during the first year of life in infants fromThe Philippines and South Africa. The study entailed the determination of the Streptococcus sp. carriage using a lytA qPCR assay, whole metagenomic sequencing, and in silico serotyping of Streptococcus pneumoniae, as well as 16S rRNA amplicon based community profiling. The lytA carriage in both populations increased with infant age and lytA+ samples ranged from 24 to 85% of the samples at each sampling time point. We next developed informatic tools for determining Streptococcus community composition and pneumococcal serotype from metagenomic sequences derived from a subset of longitudinal lytA-positive Streptococcus enrichment cultures fromThe Philippines (n = 26 infants, 50% vaccinated) and South African (n = 7 infants, 100% vaccinated). NP samples from infants were passaged in enrichment media, and metagenomic DNA was purified and sequenced. In silico capsular serotyping of these 51 metagenomic assemblies assigned known serotypes in 28 samples, and the co-occurrence of serotypes in 5 samples. Eighteen samples were not typeable using known serotypes but did encode for capsule biosynthetic cluster genes similar to non-encapsulated reference sequences. In addition, we performed metagenomic assembly and 16S rRNA amplicon profiling to understand co-colonization dynamics of Streptococcus sp. and other NP genera, revealing the presence of multiple Streptococcus species as well as potential respiratory pathogens in healthy infants. A range of virulence and drug resistant elements were identified as circulating in the NP microbiomes of these infants. This study revealed the frequent co-occurrence of multiple S. pneumoniae strains along with Streptococcus sp. and other potential pathogens such as S. aureus in the NP microbiome of these infants. In addition, the in silico serotype analysis proved powerful in determining the serotypes in S. pneumoniae carriage, and may lead to developing better targeted vaccines to prevent invasive pneumococcal disease (IPD) in these countries. These findings suggest that NP colonization by S. pneumoniae during the first years of life is a dynamic process involving multiple serotypes and species.
KW - Nasopharyngeal microbiome
KW - Pneumococcal conjugate vaccine
KW - Serotypes
KW - Streptococcus pneumoniae
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UR - http://www.scopus.com/inward/citedby.url?scp=85028959411&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2017.01661
DO - 10.3389/fmicb.2017.01661
M3 - Article
C2 - 28932211
AN - SCOPUS:85028959411
SN - 1664-302X
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - SEP
M1 - 1661
ER -