Structural Determinants of Miscibility in Surface Films of Galactosylceramide and Phosphatidylcholine: Effect of Unsaturation in the Galactosylceramide Acyl Chain

Shaukat Ali, Howard L. Brockman, Rhoderick E Brown

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The Langmuir film balance technique has been used to define the surface structure and determine the mixing behavior of galactosylceramide (GalCer) and phosphatidylcholines in surface phases. To determine the effect of unsaturation on surface behavior, chain-pure GalCer species containing either oleoyl (18:1Δ9), eicosenoyl (20:1Δ11), or eicosadienoyl (20:2Δ11, 14) fatty acyl chains were synthesized. Using bovine brain GalCer as a reference, surface pressure versus molecular area (π-A) isotherms of the pure lipids were measured and analyzed by determining their compressibilities and by using an equation of state for lipid monolayers. This information, when coupled with surface potential versus molecular area (ΔV-A) analyses, provides insights into GalCer surface structure in terms of molecular packing and orientation. Lipid mixing behavior was determined by classical approaches which involve analyzing the average molecular area, the average surface dipole moment, and surface pressure as a function of film composition. The results indicate that, in contrast to the complex mixing behavior displayed by bovine brain GalCer and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), chain-pure GalCer species containing either oleoyl, eicosenoyl, or eicosadienoyl fatty acyl chains are miscible with POPC over the entire composition range. Moreover, increasing amounts of GalCer containing eicosenoyl acyl chains systematically elevate dipalmitoyl-phosphatidylcholine’s (DPPC) liquid-expanded-to-liquid-condensed transition pressure. Such behavior is consistent with GalCer being miscible with the liquid-expanded phase of DPPC. Thus, fatty acyl unsaturation is a critical parameter governing the mixing behavior of GalCer with phosphatidylcholine.

Original languageEnglish (US)
Pages (from-to)11198-11205
Number of pages8
JournalBiochemistry
Volume30
Issue number47
DOIs
StatePublished - Nov 1 1991

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