Structural transitions of cowpea chlorotic mottle virus

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Abstract

Particles of cowpea chlorotic mottle virus (CCMV) underwent an abrupt structural transition as the pH was raised to near pH 6.75. At low ionic strength (I=0.2), the transition was observed as a decrease from 83S to 73S in the sedimentation coefficient of the virus particle. At high ionic strength (I=1.0), the virus disassembled to components sedimenting at about 40S. These observations suggest a structural role of the RNA in maintaining virus stability. As ionic strength at pH 7.5 was increased, a critical point was reached (I=0.4) at which the virus disassembled to the 40S aggregates. The effect of raising the temperature of both unswollen and swollen virus was to gradually, but substantially, reduce the sedimentation coefficient.

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalJournal of General Virology
Volume28
Issue number1
DOIs
StatePublished - 1975

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