TY - JOUR
T1 - Structural transitions of cowpea chlorotic mottle virus
AU - Adolph, K. W.
PY - 1975
Y1 - 1975
N2 - Particles of cowpea chlorotic mottle virus (CCMV) underwent an abrupt structural transition as the pH was raised to near pH 6.75. At low ionic strength (I=0.2), the transition was observed as a decrease from 83S to 73S in the sedimentation coefficient of the virus particle. At high ionic strength (I=1.0), the virus disassembled to components sedimenting at about 40S. These observations suggest a structural role of the RNA in maintaining virus stability. As ionic strength at pH 7.5 was increased, a critical point was reached (I=0.4) at which the virus disassembled to the 40S aggregates. The effect of raising the temperature of both unswollen and swollen virus was to gradually, but substantially, reduce the sedimentation coefficient.
AB - Particles of cowpea chlorotic mottle virus (CCMV) underwent an abrupt structural transition as the pH was raised to near pH 6.75. At low ionic strength (I=0.2), the transition was observed as a decrease from 83S to 73S in the sedimentation coefficient of the virus particle. At high ionic strength (I=1.0), the virus disassembled to components sedimenting at about 40S. These observations suggest a structural role of the RNA in maintaining virus stability. As ionic strength at pH 7.5 was increased, a critical point was reached (I=0.4) at which the virus disassembled to the 40S aggregates. The effect of raising the temperature of both unswollen and swollen virus was to gradually, but substantially, reduce the sedimentation coefficient.
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U2 - 10.1099/0022-1317-28-1-147
DO - 10.1099/0022-1317-28-1-147
M3 - Article
AN - SCOPUS:0016814686
SN - 0022-1317
VL - 28
SP - 147
EP - 154
JO - Journal of General Virology
JF - Journal of General Virology
IS - 1
ER -