Surface modification for protein and DNA immobilization onto GMR biosensor

Wei Wang; Yi Wang; Liang Tu; Todd Klein; Yinglong Feng; Jian Ping Wang

doi:10.1109/TMAG.2012.2224327

Surface modification for protein and DNA immobilization onto GMR biosensor

Wei Wang, Yi Wang, Liang Tu, Todd Klein, Yinglong Feng, Jian Ping Wang

Electrical and Computer Engineering

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Giant magnetoresistance (GMR) biosensor with 20 nm SiO₂ on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

Original language	English (US)
Article number	6392433
Pages (from-to)	296-299
Number of pages	4
Journal	IEEE Transactions on Magnetics
Volume	49
Issue number	1
DOIs	https://doi.org/10.1109/TMAG.2012.2224327
State	Published - 2013

Keywords

Deoxyribonucleic acid (DNA)
giant magnetoresistance (GMR) biosensor
immobilization
protein
surface modification

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title = "Surface modification for protein and DNA immobilization onto GMR biosensor",

abstract = "Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.",

keywords = "Deoxyribonucleic acid (DNA), giant magnetoresistance (GMR) biosensor, immobilization, protein, surface modification",

author = "Wei Wang and Yi Wang and Liang Tu and Todd Klein and Yinglong Feng and Wang, {Jian Ping}",

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AU - Wang, Wei

AU - Wang, Yi

AU - Tu, Liang

AU - Klein, Todd

AU - Feng, Yinglong

AU - Wang, Jian Ping

PY - 2013

Y1 - 2013

N2 - Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

AB - Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

KW - Deoxyribonucleic acid (DNA)

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KW - immobilization

KW - protein

KW - surface modification

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Surface modification for protein and DNA immobilization onto GMR biosensor

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