Surface modification for protein and DNA immobilization onto GMR biosensor

Wei Wang, Yi Wang, Liang Tu, Todd Klein, Yinglong Feng, Jianping Wang

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

Original languageEnglish (US)
Article number6392433
Pages (from-to)296-299
Number of pages4
JournalIEEE Transactions on Magnetics
Volume49
Issue number1
DOIs
StatePublished - Jan 4 2013

Keywords

  • Deoxyribonucleic acid (DNA)
  • giant magnetoresistance (GMR) biosensor
  • immobilization
  • protein
  • surface modification

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