To study the binding sites of protein prenyltransferases, we have synthesized photoaffinity labeling analogs that contain photoactive benzophenone units. Here we describe the syntheses and a method for the rapid purification of two 32P-labeled analogs; phosphorylation was accomplished by reacting the corresponding alcohols with CCl3CN and [32P]-H3PO4 in CH3CN and the purification was performed using C18 reversed-phase cartridges. Analog [32P]-3a was prepared from 2.4 mg of starting alcohol 4a and was obtained in 3.3% yield, 50% radiochemical purity and 480 Ci/mol specific activity. Further purification by preparative thin layer chromatography yielded material with a radiochemical purity of 90%. A second analog, [32P]-3b, was prepared from 1.2 mg of alcohol 4b and was obtained in 1.0% yield, 54% radiochemical purity and 700 Ci/mol specific activity. Further purification by preparative thin layer chromatography yielded material with a radiochemical purity of 91%.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Labelled Compounds and Radiopharmaceuticals|
|State||Published - Feb 1 1997|
- Farnesyl pyrophosphate analog
- Geranylgeranyl pyrophosphate analog
- Photoaffinity labeling