TY - JOUR
T1 - Tandem use of X-ray crystallography and mass spectrometry to obtain ab initio the complete and exact amino acids sequence of HPBP, a human 38-kDa apolipoprotein
AU - Diemer, Hélène
AU - Elias, Mikael
AU - Renault, Frédérique
AU - Rochu, Daniel
AU - Contreras-Martel, Carlos
AU - Schaeffer, Christine
AU - Van Dorsselaer, Alain
AU - Chabriere, Eric
PY - 2008/6
Y1 - 2008/6
N2 - The Human Phosphate Binding Protein (HPBP) is a serendipitously discovered apolipoprotein from human plasma that binds phosphate. Amino acid sequence relates HPBP to an intriguing protein family that seems ubiquitous in eukaryotes. These proteins, named DING according to the sequence of their four conserved N-terminal residues, are systematically absent from eukaryotic genome databases. As a consequence, HPBP amino acids sequence had to be first assigned from the electronic density map. Then, an original approach combining X-ray crystallography and mass spectrometry provides the complete and a priori exact sequence of the 38-kDa HPBP. This first complete sequence of a eukaryotic DING protein will be helpful to study HPBP and the entire DING protein family.
AB - The Human Phosphate Binding Protein (HPBP) is a serendipitously discovered apolipoprotein from human plasma that binds phosphate. Amino acid sequence relates HPBP to an intriguing protein family that seems ubiquitous in eukaryotes. These proteins, named DING according to the sequence of their four conserved N-terminal residues, are systematically absent from eukaryotic genome databases. As a consequence, HPBP amino acids sequence had to be first assigned from the electronic density map. Then, an original approach combining X-ray crystallography and mass spectrometry provides the complete and a priori exact sequence of the 38-kDa HPBP. This first complete sequence of a eukaryotic DING protein will be helpful to study HPBP and the entire DING protein family.
KW - Amino acids sequencing
KW - Atherosclerosis
KW - DING protein
KW - Human phosphate binding protein
KW - Mass spectrometry
KW - Missing gene
KW - X-ray crystallography
UR - http://www.scopus.com/inward/record.url?scp=44349106522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44349106522&partnerID=8YFLogxK
U2 - 10.1002/prot.21866
DO - 10.1002/prot.21866
M3 - Article
C2 - 18076037
AN - SCOPUS:44349106522
SN - 0887-3585
VL - 71
SP - 1708
EP - 1720
JO - Proteins: Structure, Function and Genetics
JF - Proteins: Structure, Function and Genetics
IS - 4
ER -