Abstract
Isolated muscle fibers from Rana temporaria tibialis anterior muscles were microinjected with aequorin. The force responses and the Ca2+ transients associated with twitch and tetanic contractions were studied at several temperatures. The declines of the Ca2+ transients were well described by single exponential equations and the effects of temperature were complex (multi-exponential). To determine if these temperature effects on the Ca2+ transients were influenced by the Ca2+ indicator itself, samples of the injected aequorin were studied in vitro using a Gibson stopped-flow apparatus. The quenching of aequorin luminescence with either EGTA or de-calcified Rana temporaria parvalbumin were mono-exponential. These overall quenching reactions had single exponential temperature dependencies. The effects of temperature on the declines of the single fiber Ca2+ transients did not appear to be influenced by the kinetics of the aequorin reaction. The disparity in the effects of temperature on the single fiber Ca2+ transients versus the in vitro quenching of aequorin luminescence with parvalbumin, were interpreted to indicate that in twitch and tetanic contractions of these fibers, it was unlikely that soluble Ca2+ binding proteins played a major role in the regulation of myoplasmic Ca2+.
Original language | English (US) |
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Pages (from-to) | 195-202 |
Number of pages | 8 |
Journal | Pflügers Archiv European Journal of Physiology |
Volume | 412 |
Issue number | 1-2 |
DOIs | |
State | Published - Jul 1 1988 |
Keywords
- Aequorin
- Single fibers
- Stopped-flow quenching
- Tetani
- Twitches