TY - JOUR
T1 - The genes encoding the Eph-related receptor tyrosine kinase ligands LERK-1 (EPLG1, Epl1), LERK-3 (EPLG3, Epl3), and LERK-4 (EPLG4, Epl4) are clustered on human chromosome 1 and mouse chromosome 3
AU - Cerretti, Douglas Pat
AU - Lyman, Stewart D.
AU - Kozlosky, Carl J.
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
AU - Valentine, Virginia
AU - Kirstein, Mark N.
AU - Shapiro, David N.
AU - Morris, Stephan W.
N1 - Funding Information:
This work was supported in part by National Cancer Institute (NCI) Grant CA01702 (S.W.M.), Cancer Center CORE Grants CA21765 and CA23099, the American Lebanese Syrian Associated Charities (ALSAC), and the National Cancer Institute, DHHS, under Contract NO1-CO-74101 with ABL (N.G.C., D.J.G., and N.A.J.).
PY - 1996/4/15
Y1 - 1996/4/15
N2 - Hek and elk are members of the eph-related family of receptor tyrosine kinases. Recently, we isolated five cDNAs encoding membrane-bound ligands to hek and elk. Because of the promiscuous nature of their binding, we have termed these proteins ligands of the eph-related kinases or LERKs. The LERKs can be divided into two subgroups by virtue of their sequence identity, binding properties, and mode of cell membrane attachment. For example, LERK-2 (EPLG2, Epl2) and LERK-5 (EPLG5, Epl5) are type 1 transmembrane proteins, while LERK-1 (EPLG1, Epl1), LERK-3 (EPLG3, Epl3), and LERK-4 (EPLG4, Epl4) are anchored to the membrane by glycosyl-phosphatidylinositol (GPI) linkage. Using Southern hybridization analysis of human X rodent somatic cell hybrid DNAs, we have assigned the genes that encode the GPI-anchored LERKs (EPLG1, EPLG3, and EPLG4) to human chromosome 1. Fluorescence in situ hybridization to metaphase chromosome preparations using genomic clones from each locus refined this localization to chromosome 1, bands q21-q22. In addition, Southern blot analysis of DNA from interspecific backcross mice indicated that the mouse homologues Epl1, Epl3, and Epl4 map to a homologous region on mouse chromosome 3.
AB - Hek and elk are members of the eph-related family of receptor tyrosine kinases. Recently, we isolated five cDNAs encoding membrane-bound ligands to hek and elk. Because of the promiscuous nature of their binding, we have termed these proteins ligands of the eph-related kinases or LERKs. The LERKs can be divided into two subgroups by virtue of their sequence identity, binding properties, and mode of cell membrane attachment. For example, LERK-2 (EPLG2, Epl2) and LERK-5 (EPLG5, Epl5) are type 1 transmembrane proteins, while LERK-1 (EPLG1, Epl1), LERK-3 (EPLG3, Epl3), and LERK-4 (EPLG4, Epl4) are anchored to the membrane by glycosyl-phosphatidylinositol (GPI) linkage. Using Southern hybridization analysis of human X rodent somatic cell hybrid DNAs, we have assigned the genes that encode the GPI-anchored LERKs (EPLG1, EPLG3, and EPLG4) to human chromosome 1. Fluorescence in situ hybridization to metaphase chromosome preparations using genomic clones from each locus refined this localization to chromosome 1, bands q21-q22. In addition, Southern blot analysis of DNA from interspecific backcross mice indicated that the mouse homologues Epl1, Epl3, and Epl4 map to a homologous region on mouse chromosome 3.
UR - http://www.scopus.com/inward/record.url?scp=0029990095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029990095&partnerID=8YFLogxK
U2 - 10.1006/geno.1996.0192
DO - 10.1006/geno.1996.0192
M3 - Article
C2 - 8660976
AN - SCOPUS:0029990095
SN - 0888-7543
VL - 33
SP - 277
EP - 282
JO - Genomics
JF - Genomics
IS - 2
ER -