Physical and genetic maps have been constructed for mtDNA from strains of the fungus Fusarium oxysporum representing three pathogenically specialized forms. All three mtDNA maps are circular. Their sizes are 45 kb for F. oxysporum f.sp. raphani and 52 kb for both F. oxysporum f.sp. conglutinans and F. oxysporum f.sp. matthioli. The genetic loci for cytochrome b, the mitochondrial 25S ribosomal RNA and cytochrome oxidase subunit II, have been identified and are similarly arranged on the three genomes.
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We thankS ally Leong,P aul Williams,a nd Paul Bosland for initial supporta nd encouragemefnotr thisw ork.F rank Martin and ErnestH ieberta rer ecognizedfo r their review ofthem anuscripat ndJ ohn Taylori s thankedf or providing clonesm entionedi n the text. This work was supported by the Instituteo f Food and AgriculturalS ciencesU, ni-versityo f Florida, and USDA Grant 86-CRCR-l-2 195.