TY - JOUR
T1 - The MMTV LTR promoter is induced by progesterone and dihydrotestosterone but not by estrogen
AU - Otten, Anne D.
AU - Sanders, Michel M.
AU - McKnight, G. Stanley
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/8
Y1 - 1988/8
N2 - Induction of the mouse mammary tumor virus (MMTV) promoter by steroid hormones was examined in chick oviduct primary cell cultures transfected with MMTV-chloramphenicol acetyl transferase fusion constructs. Our results demonstrate that in this system glucocorticoids, progesterone and dihydrotestosterone are all able to stimulate MMTV transcription; induction by progesterone and dihydrotestosterone is not mediated by glucocorticoid receptors, since the specific glucocorticoid antagonist RU486 did not inhibit the response. In contrast, estrogen does not stimulate MMTV transcription, although estrogen does induce the endogenous ovalbumin gene in the same cells. While progesterone effects are mediated by the same response elements within the MMTV long terminal repeat that were originally characterized for the glucocorticoid receptor, an androgen response element has not yet been defined. Our data indicate that the MMTV long terminal repeat does not contain an estrogen response element.
AB - Induction of the mouse mammary tumor virus (MMTV) promoter by steroid hormones was examined in chick oviduct primary cell cultures transfected with MMTV-chloramphenicol acetyl transferase fusion constructs. Our results demonstrate that in this system glucocorticoids, progesterone and dihydrotestosterone are all able to stimulate MMTV transcription; induction by progesterone and dihydrotestosterone is not mediated by glucocorticoid receptors, since the specific glucocorticoid antagonist RU486 did not inhibit the response. In contrast, estrogen does not stimulate MMTV transcription, although estrogen does induce the endogenous ovalbumin gene in the same cells. While progesterone effects are mediated by the same response elements within the MMTV long terminal repeat that were originally characterized for the glucocorticoid receptor, an androgen response element has not yet been defined. Our data indicate that the MMTV long terminal repeat does not contain an estrogen response element.
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U2 - 10.1210/mend-2-2-143
DO - 10.1210/mend-2-2-143
M3 - Article
C2 - 2840570
AN - SCOPUS:0023874442
SN - 0888-8809
VL - 2
SP - 143
EP - 147
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 2
ER -