The photolysis of 5′-deoxyadenosylcobalamin under anaerobic conditions

P. Y. Law; J. M. Wood

doi:10.1016/0005-2787(73)90032-4

The photolysis of 5′-deoxyadenosylcobalamin under anaerobic conditions

P. Y. Law, J. M. Wood

Research output: Contribution to journal › Article › peer-review

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Abstract

Nuclear magnetic resonance and mass spectrometry have been used to confirm that the nucleoside product formed when 5′-deoxyadenosylcobalamin is photolysed under anaerobic conditions is 8,5′-cyclic adenosine. The synthesis of 5′-deoxyadenosylcobalamin, which is tritiated at C₈ of the adenosyl moiety, provides the confirmation that the photolysis mechanism involves a reaction between the 5′-methylene carbon radical and C₈ of the adenine ring.

Original language	English (US)
Pages (from-to)	451-454
Number of pages	4
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	331
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(73)90032-4
State	Published - Dec 21 1973
Externally published	Yes

Bibliographical note

Funding Information:
The mass spectrum of the nucleoside product is shown in Fig. 2. The molecular ion is at m/e 249 which corresponds to the formula CloNs Hl103. The structure is substantiated as 8,5 '-cyclic adenosine by the fragmentation pattern (Scheme I). Peaks of m/e 249, 192, 177, 163,149, t35 and 108 are accounted for by this fragmentation scheme. These data confirm that the structure of the nucleoside photolysis product from 5'-deoxyadenosylcobalamin is 8,5'-cyclic adenosine, and support a mechanism of Y-carbon radical attack at C8. This research was supported by a grant from the U.S. Public Health Service AM

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title = "The photolysis of 5′-deoxyadenosylcobalamin under anaerobic conditions",

abstract = "Nuclear magnetic resonance and mass spectrometry have been used to confirm that the nucleoside product formed when 5′-deoxyadenosylcobalamin is photolysed under anaerobic conditions is 8,5′-cyclic adenosine. The synthesis of 5′-deoxyadenosylcobalamin, which is tritiated at C8 of the adenosyl moiety, provides the confirmation that the photolysis mechanism involves a reaction between the 5′-methylene carbon radical and C8 of the adenine ring.",

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note = "Funding Information: The mass spectrum of the nucleoside product is shown in Fig. 2. The molecular ion is at m/e 249 which corresponds to the formula CloNs Hl103. The structure is substantiated as 8,5 '-cyclic adenosine by the fragmentation pattern (Scheme I). Peaks of m/e 249, 192, 177, 163,149, t35 and 108 are accounted for by this fragmentation scheme. These data confirm that the structure of the nucleoside photolysis product from 5'-deoxyadenosylcobalamin is 8,5'-cyclic adenosine, and support a mechanism of Y-carbon radical attack at C8. This research was supported by a grant from the U.S. Public Health Service AM",

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N1 - Funding Information: The mass spectrum of the nucleoside product is shown in Fig. 2. The molecular ion is at m/e 249 which corresponds to the formula CloNs Hl103. The structure is substantiated as 8,5 '-cyclic adenosine by the fragmentation pattern (Scheme I). Peaks of m/e 249, 192, 177, 163,149, t35 and 108 are accounted for by this fragmentation scheme. These data confirm that the structure of the nucleoside photolysis product from 5'-deoxyadenosylcobalamin is 8,5'-cyclic adenosine, and support a mechanism of Y-carbon radical attack at C8. This research was supported by a grant from the U.S. Public Health Service AM

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N2 - Nuclear magnetic resonance and mass spectrometry have been used to confirm that the nucleoside product formed when 5′-deoxyadenosylcobalamin is photolysed under anaerobic conditions is 8,5′-cyclic adenosine. The synthesis of 5′-deoxyadenosylcobalamin, which is tritiated at C8 of the adenosyl moiety, provides the confirmation that the photolysis mechanism involves a reaction between the 5′-methylene carbon radical and C8 of the adenine ring.

AB - Nuclear magnetic resonance and mass spectrometry have been used to confirm that the nucleoside product formed when 5′-deoxyadenosylcobalamin is photolysed under anaerobic conditions is 8,5′-cyclic adenosine. The synthesis of 5′-deoxyadenosylcobalamin, which is tritiated at C8 of the adenosyl moiety, provides the confirmation that the photolysis mechanism involves a reaction between the 5′-methylene carbon radical and C8 of the adenine ring.

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The photolysis of 5′-deoxyadenosylcobalamin under anaerobic conditions

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