TY - JOUR
T1 - The regulated expression of a diverse set of genes during thymocyte positive selection in vivo
AU - Mick, Verity E.
AU - Starr, Timothy K.
AU - McCaughtry, Tom M.
AU - McNeil, Lisa K.
AU - Hogquist, Kristin A.
PY - 2004/11/1
Y1 - 2004/11/1
N2 - A signal initiated by the newly formed Ag receptor is integrated with microenvironmcntal cues during T cell development to ensure positive selection of CD4+CD8+ progenitors into functionally mature CD4 + or CD8+ T lymphocytes. During this transition, a survival program is initiated, TCR gene recombination ceases, cells migrate into a new thymic microenvironment, the responsiveness of the Ag receptor is tuned, and the cells commit to a specific T lineage. To determine potential regulators of these processes, we used mRNA microarray analysis to compare gens expression changes in CD4+CD8+ thymocytes from TCR transgenic mice that have received a TCR selection signal with those that had not received a signal. We found 129 genes with expression that changed significantly during positive selection, the majority of which were not previously appreciated. A large number of these changes were confirmed by real-time PCR or flow cytometry. We have combined our findings with gene changes reported in the literature to provide a comprehensive report of the genes regulated during positive selection, and we attempted to assign these genes to positive selection process categories.
AB - A signal initiated by the newly formed Ag receptor is integrated with microenvironmcntal cues during T cell development to ensure positive selection of CD4+CD8+ progenitors into functionally mature CD4 + or CD8+ T lymphocytes. During this transition, a survival program is initiated, TCR gene recombination ceases, cells migrate into a new thymic microenvironment, the responsiveness of the Ag receptor is tuned, and the cells commit to a specific T lineage. To determine potential regulators of these processes, we used mRNA microarray analysis to compare gens expression changes in CD4+CD8+ thymocytes from TCR transgenic mice that have received a TCR selection signal with those that had not received a signal. We found 129 genes with expression that changed significantly during positive selection, the majority of which were not previously appreciated. A large number of these changes were confirmed by real-time PCR or flow cytometry. We have combined our findings with gene changes reported in the literature to provide a comprehensive report of the genes regulated during positive selection, and we attempted to assign these genes to positive selection process categories.
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U2 - 10.4049/jimmunol.173.9.5434
DO - 10.4049/jimmunol.173.9.5434
M3 - Article
C2 - 15494490
AN - SCOPUS:6344274985
SN - 0022-1767
VL - 173
SP - 5434
EP - 5444
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -