Abstract
A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30°C to 45°C, and the respiration that resumed later at 45°C was cyanide-insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply in cells at 45°C. Electron microscopy showed no changes in mitochondrial complexity; however, the cytoplasm of heat-shocked cells was deficient in glycogen granules.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-322 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 81 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1 1991 |
Bibliographical note
Funding Information:This research was supported by grants from the National Institute of General Medical Sciences (GM-19398) and the United States Depart- merit of Agriculture Competitive Research Grants Office (88-37264-4045).
Keywords
- Glycogen
- Heat shock
- Microscopy
- Mitochondria
- Neurospora crassa
- Respiration