A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30°C to 45°C, and the respiration that resumed later at 45°C was cyanide-insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply in cells at 45°C. Electron microscopy showed no changes in mitochondrial complexity; however, the cytoplasm of heat-shocked cells was deficient in glycogen granules.
|Original language||English (US)|
|Number of pages||6|
|Journal||FEMS Microbiology Letters|
|State||Published - Jul 1 1991|
Bibliographical noteFunding Information:
This research was supported by grants from the National Institute of General Medical Sciences (GM-19398) and the United States Depart- merit of Agriculture Competitive Research Grants Office (88-37264-4045).
Copyright 2018 Elsevier B.V., All rights reserved.
- Heat shock
- Neurospora crassa