TY - JOUR
T1 - The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250
AU - Shao, Zhaohui
AU - Ruppert, Siegfried
AU - Robbins, Paul D.
PY - 1995/4/11
Y1 - 1995/4/11
N2 - RB, the protein product of the retinoblastoma tumor-suppressor gene, regulates the activity of specific transcription factors. This regulation appears to be mediated either directly through interactions with specific transcription factors or through an alternative mechanism. Here we report that stimulation of Sp1-mediated transcription by RB is partially abrogated at the nonpermissive temperature in ts13 cells. These cells contain a temperature-sensitive mutation in the TATA-binding protein-associated factor TAFII250, first identified as the cell cycle regulatory protein CCG1. The stimulation of Sp1-mediated transcription by RB in ts13 cells at the nonpermissive temperature could be restored by the introduction of wild-type human TAFII250. Furthermore, we demonstrate that RB binds directly to hTAFII250 in vitro and in vivo. These results suggest that RB can confer transcriptional regulation and possibly cell cycle control and tumor suppression through an interaction with TFIID, in particular with TAFII250.
AB - RB, the protein product of the retinoblastoma tumor-suppressor gene, regulates the activity of specific transcription factors. This regulation appears to be mediated either directly through interactions with specific transcription factors or through an alternative mechanism. Here we report that stimulation of Sp1-mediated transcription by RB is partially abrogated at the nonpermissive temperature in ts13 cells. These cells contain a temperature-sensitive mutation in the TATA-binding protein-associated factor TAFII250, first identified as the cell cycle regulatory protein CCG1. The stimulation of Sp1-mediated transcription by RB in ts13 cells at the nonpermissive temperature could be restored by the introduction of wild-type human TAFII250. Furthermore, we demonstrate that RB binds directly to hTAFII250 in vitro and in vivo. These results suggest that RB can confer transcriptional regulation and possibly cell cycle control and tumor suppression through an interaction with TFIID, in particular with TAFII250.
KW - Protein-protein interaction
KW - Transcription factor TFIID
UR - http://www.scopus.com/inward/record.url?scp=0028932792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028932792&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.8.3115
DO - 10.1073/pnas.92.8.3115
M3 - Article
C2 - 7724524
AN - SCOPUS:0028932792
SN - 0027-8424
VL - 92
SP - 3115
EP - 3119
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -