Tisagenlecleucel in adult relapsed or refractory diffuse large B-cell lymphoma

JULIET Investigators

Research output: Contribution to journalArticlepeer-review

550 Scopus citations


Background: Patients with diffuse large B-cell lymphoma that is refractory to primary and secondline therapies or that has relapsed after stem-cell transplantation have a poor prognosis. The chimeric antigen receptor (CAR) T-cell therapy tisagenlecleucel targets and eliminates CD19-expressing B cells and showed efficacy against B-cell lymphomas in a single-center, phase 2a study. Methods: We conducted an international, phase 2, pivotal study of centrally manufactured tisagenlecleucel involving adult patients with relapsed or refractory diffuse large B-cell lymphoma who were ineligible for or had disease progression after autologous hematopoietic stem-cell transplantation. The primary end point was the best overall response rate (i.e., the percentage of patients who had a complete or partial response), as judged by an independent review committee. Results: A total of 93 patients received an infusion and were included in the evaluation of efficacy. The median time from infusion to data cutoff was 14 months (range, 0.1 to 26). The best overall response rate was 52% (95% confidence interval, 41 to 62); 40% of the patients had complete responses, and 12% had partial responses. Response rates were consistent across prognostic subgroups. At 12 months after the initial response, the rate of relapse-free survival was estimated to be 65% (79% among patients with a complete response). The most common grade 3 or 4 adverse events of special interest included cytokine release syndrome (22%), neurologic events (12%), cytopenias lasting more than 28 days (32%), infections (20%), and febrile neutropenia (14%). Three patients died from disease progression within 30 days after infusion. No deaths were attributed to tisagenlecleucel, cytokine release syndrome, or cerebral edema. No differences between response groups in tumor expression of CD19 or immune checkpoint- related proteins were found. Conclusions: In this international study of CAR T-cell therapy in relapsed or refractory diffuse large B-cell lymphoma in adults, high rates of durable responses were produced with the use of tisagenlecleucel.

Original languageEnglish (US)
Pages (from-to)45-56
Number of pages12
JournalNew England Journal of Medicine
Issue number1
StatePublished - Jan 3 2019

Bibliographical note

Funding Information:
The study was sponsored and designed by No-vartis and was approved by the institutional review board at each participating institution. Data were analyzed and interpreted by the sponsor and the authors. All the authors reviewed the manuscript. The authors vouch for the data and analysis and for the adherence of the study to the protocol, which is available with the full text of this article at NEJM.org. Editorial assistance with the preparation of the manuscript for submission was financially supported by Novartis.

Funding Information:
Supported by Novartis. Editorial assistance with an earlier version of the manuscript was provided by ArticulateScience and paid for by Novartis.

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