Tissue factor gene expression in acute myeloblastic leukemia

Kenneth A. Bauer; Edward M. Conway; Ronald R Bach; William H. Konigsberg; James D. Griffin; George Demetri

doi:10.1016/0049-3848(89)90255-7

Tissue factor gene expression in acute myeloblastic leukemia

Kenneth A. Bauer, Edward M. Conway, Ronald R Bach, William H. Konigsberg, James D. Griffin, George Demetri

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We have studied tissue factor gene expression in the leukocytes of 22 patients with acute myeloblastic leukemia (AML). Total RNA from peripheral blood or bone marrow cells depleted of monocytes was analyzed by Northern blot analysis using a ³²p-labeled tissue factor cDNA probe. Cells from 10 cases expressed tissue factor mRNA and positive cases were distributed among the myeloblastic, myelomonocytic, and monocytic subtypes of AML. Tissue factor transcripts were not detected in cells derived from normal bone marrow. The expression of this gene product on the surface of leukemic cells could contribute to the excessive thrombin generation that has been observed in some individuals with this disorder.

Original language	English (US)
Pages (from-to)	425-430
Number of pages	6
Journal	Thrombosis Research
Volume	56
Issue number	3
DOIs	https://doi.org/10.1016/0049-3848(89)90255-7
State	Published - Nov 1 1989

Bibliographical note

Funding Information:
This study was supported in part by grants PO1 HL 33014 and PO1 CA 34183 from the National Institutes of Health.

Keywords

Tissue factor
complementary DNA
messenger RNA
myeloblastic leukemia

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title = "Tissue factor gene expression in acute myeloblastic leukemia",

abstract = "We have studied tissue factor gene expression in the leukocytes of 22 patients with acute myeloblastic leukemia (AML). Total RNA from peripheral blood or bone marrow cells depleted of monocytes was analyzed by Northern blot analysis using a 32p-labeled tissue factor cDNA probe. Cells from 10 cases expressed tissue factor mRNA and positive cases were distributed among the myeloblastic, myelomonocytic, and monocytic subtypes of AML. Tissue factor transcripts were not detected in cells derived from normal bone marrow. The expression of this gene product on the surface of leukemic cells could contribute to the excessive thrombin generation that has been observed in some individuals with this disorder.",

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AU - Bauer, Kenneth A.

AU - Conway, Edward M.

AU - Bach, Ronald R

AU - Konigsberg, William H.

AU - Griffin, James D.

AU - Demetri, George

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KW - Tissue factor

KW - complementary DNA

KW - messenger RNA

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Tissue factor gene expression in acute myeloblastic leukemia

Abstract

Bibliographical note

Keywords

UN SDGs

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