Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

Yi Cong; Gary T. Banta; Henriette Selck; Deborah Berhanu; Eugenia Valsami-Jones; Valery E. Forbes

doi:10.1016/j.aquatox.2011.07.014

Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

Yi Cong, Gary T. Banta, Henriette Selck, Deborah Berhanu, Eugenia Valsami-Jones, Valery E. Forbes

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release via wastewater discharges. In this study, the toxicities of sediment associated nano (<100nm)-, micron (2-3.5μm)- and ionic (AgNO ₃)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 days of sediment exposure, using survival, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, and 50μgAg/g dry weight (dw) sediment. Our results showed that Ag was able to cause DNA damage in Nereis coelomocytes, and that this effect was both concentration- and Ag form-related. There was significantly greater genotoxicity (higher tail moment and tail DNA intensities) at 25 and 50μg/gdw in nano- and micron-Ag treatments and at 50μg/gdw in the ionic-Ag treatment compared to the controls (0μg/gdw). The nano-Ag treatment had the greatest genotoxic effect of the three tested Ag forms, and the ionic-Ag treatment was the least genotoxic. N. diversicolor did accumulate sediment-associated Ag from all three forms. Ag body burdens at the highest exposure concentration were 8.56±6.63, 6.92±5.86 and 9.86±4.94μg/gdw for worms in nano-, micron- and ionic-Ag treatments, respectively, but there was no significant difference in Ag bioaccumulation among the three treatments.

Original language	English (US)
Pages (from-to)	403-411
Number of pages	9
Journal	Aquatic Toxicology
Volume	105
Issue number	3-4
DOIs	https://doi.org/10.1016/j.aquatox.2011.07.014
State	Published - Oct 2011
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Roskilde University (RUC) and is done in cooperation with the EU NanoReTox project (7th Framework Programme, Grant Agreement no. CP-FP 214478-2). The authors gratefully acknowledge Anne-Grete Winding, Klara Jensen, Anja Holden Damsholt, Anne Busk Faarborg and Rikke Guttesen for their technical guidance and assistance during experiments. Support from the China Scholarship Council (CSC) is also gratefully acknowledged.

Keywords

Ag body burden
Genotoxicity
Marine invertebrate
Sediment exposure
Silver nanoparticles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1016/j.aquatox.2011.07.014

OpenUrl availability

Full text

Cite this

@article{9a51ce3abbd641a1b274e3f422af4af9,

title = "Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor",

abstract = "There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release via wastewater discharges. In this study, the toxicities of sediment associated nano (<100nm)-, micron (2-3.5μm)- and ionic (AgNO 3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 days of sediment exposure, using survival, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, and 50μgAg/g dry weight (dw) sediment. Our results showed that Ag was able to cause DNA damage in Nereis coelomocytes, and that this effect was both concentration- and Ag form-related. There was significantly greater genotoxicity (higher tail moment and tail DNA intensities) at 25 and 50μg/gdw in nano- and micron-Ag treatments and at 50μg/gdw in the ionic-Ag treatment compared to the controls (0μg/gdw). The nano-Ag treatment had the greatest genotoxic effect of the three tested Ag forms, and the ionic-Ag treatment was the least genotoxic. N. diversicolor did accumulate sediment-associated Ag from all three forms. Ag body burdens at the highest exposure concentration were 8.56±6.63, 6.92±5.86 and 9.86±4.94μg/gdw for worms in nano-, micron- and ionic-Ag treatments, respectively, but there was no significant difference in Ag bioaccumulation among the three treatments.",

keywords = "Ag body burden, Genotoxicity, Marine invertebrate, Sediment exposure, Silver nanoparticles",

author = "Yi Cong and Banta, {Gary T.} and Henriette Selck and Deborah Berhanu and Eugenia Valsami-Jones and Forbes, {Valery E.}",

note = "Funding Information: This work was supported by Roskilde University (RUC) and is done in cooperation with the EU NanoReTox project (7th Framework Programme, Grant Agreement no. CP-FP 214478-2). The authors gratefully acknowledge Anne-Grete Winding, Klara Jensen, Anja Holden Damsholt, Anne Busk Faarborg and Rikke Guttesen for their technical guidance and assistance during experiments. Support from the China Scholarship Council (CSC) is also gratefully acknowledged.",

year = "2011",

month = oct,

doi = "10.1016/j.aquatox.2011.07.014",

language = "English (US)",

volume = "105",

pages = "403--411",

journal = "Aquatic Toxicology",

issn = "0166-445X",

publisher = "Elsevier",

number = "3-4",

}

TY - JOUR

T1 - Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

AU - Cong, Yi

AU - Banta, Gary T.

AU - Selck, Henriette

AU - Berhanu, Deborah

AU - Valsami-Jones, Eugenia

AU - Forbes, Valery E.

N1 - Funding Information: This work was supported by Roskilde University (RUC) and is done in cooperation with the EU NanoReTox project (7th Framework Programme, Grant Agreement no. CP-FP 214478-2). The authors gratefully acknowledge Anne-Grete Winding, Klara Jensen, Anja Holden Damsholt, Anne Busk Faarborg and Rikke Guttesen for their technical guidance and assistance during experiments. Support from the China Scholarship Council (CSC) is also gratefully acknowledged.

PY - 2011/10

Y1 - 2011/10

N2 - There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release via wastewater discharges. In this study, the toxicities of sediment associated nano (<100nm)-, micron (2-3.5μm)- and ionic (AgNO 3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 days of sediment exposure, using survival, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, and 50μgAg/g dry weight (dw) sediment. Our results showed that Ag was able to cause DNA damage in Nereis coelomocytes, and that this effect was both concentration- and Ag form-related. There was significantly greater genotoxicity (higher tail moment and tail DNA intensities) at 25 and 50μg/gdw in nano- and micron-Ag treatments and at 50μg/gdw in the ionic-Ag treatment compared to the controls (0μg/gdw). The nano-Ag treatment had the greatest genotoxic effect of the three tested Ag forms, and the ionic-Ag treatment was the least genotoxic. N. diversicolor did accumulate sediment-associated Ag from all three forms. Ag body burdens at the highest exposure concentration were 8.56±6.63, 6.92±5.86 and 9.86±4.94μg/gdw for worms in nano-, micron- and ionic-Ag treatments, respectively, but there was no significant difference in Ag bioaccumulation among the three treatments.

AB - There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release via wastewater discharges. In this study, the toxicities of sediment associated nano (<100nm)-, micron (2-3.5μm)- and ionic (AgNO 3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 days of sediment exposure, using survival, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, and 50μgAg/g dry weight (dw) sediment. Our results showed that Ag was able to cause DNA damage in Nereis coelomocytes, and that this effect was both concentration- and Ag form-related. There was significantly greater genotoxicity (higher tail moment and tail DNA intensities) at 25 and 50μg/gdw in nano- and micron-Ag treatments and at 50μg/gdw in the ionic-Ag treatment compared to the controls (0μg/gdw). The nano-Ag treatment had the greatest genotoxic effect of the three tested Ag forms, and the ionic-Ag treatment was the least genotoxic. N. diversicolor did accumulate sediment-associated Ag from all three forms. Ag body burdens at the highest exposure concentration were 8.56±6.63, 6.92±5.86 and 9.86±4.94μg/gdw for worms in nano-, micron- and ionic-Ag treatments, respectively, but there was no significant difference in Ag bioaccumulation among the three treatments.

KW - Ag body burden

KW - Genotoxicity

KW - Marine invertebrate

KW - Sediment exposure

KW - Silver nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=79961125585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961125585&partnerID=8YFLogxK

U2 - 10.1016/j.aquatox.2011.07.014

DO - 10.1016/j.aquatox.2011.07.014

M3 - Article

C2 - 21831346

AN - SCOPUS:79961125585

SN - 0166-445X

VL - 105

SP - 403

EP - 411

JO - Aquatic Toxicology

JF - Aquatic Toxicology

IS - 3-4

ER -

Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

Abstract

Bibliographical note

Keywords

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this