Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe

Santos Angel, Hedley C. Freake, Mark E. Rosenberg, Harold L. Schwartz, Jack H. Oppenheimer

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Recent studies have raised the possibility of multiple structurally distinctive tissue-specific nuclear T3 receptors, all exhibiting homology with the v-erbA oncogene and represented by mRNAs of various sizes. We have assayed the level of mRNAs recognized by a32P-labeled cRNA derived from human plancetal c-erbA-Aβ cDNA by solution hybridization and by Northern transfer in different rat tissues, as well as human liver and placenta. Two related mRNAs were demonstrated in the rat tissues analyzed, one measuring 6.0 and the other 2.6 kilobases (kb). In human liver and placenta a 6.0 kb mRNA was seen, but not a 2.6 kb mRNA. Only the 6.0 kb sequence correlated with the receptor concentration determined by125I-T3 displacement analysis.

Original languageEnglish (US)
Pages (from-to)992-998
Number of pages7
JournalMolecular Endocrinology
Volume2
Issue number10
DOIs
StatePublished - Oct 1988

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