Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe

Santos Angel; Hedley C. Freake; Mark E. Rosenberg; Harold L. Schwartz; Jack H. Oppenheimer

doi:10.1210/mend-2-10-992

Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe

Santos Angel, Hedley C. Freake, Mark E. Rosenberg, Harold L. Schwartz, Jack H. Oppenheimer

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Abstract

Recent studies have raised the possibility of multiple structurally distinctive tissue-specific nuclear T₃ receptors, all exhibiting homology with the v-erbA oncogene and represented by mRNAs of various sizes. We have assayed the level of mRNAs recognized by a³²P-labeled cRNA derived from human plancetal c-erbA-Aβ cDNA by solution hybridization and by Northern transfer in different rat tissues, as well as human liver and placenta. Two related mRNAs were demonstrated in the rat tissues analyzed, one measuring 6.0 and the other 2.6 kilobases (kb). In human liver and placenta a 6.0 kb mRNA was seen, but not a 2.6 kb mRNA. Only the 6.0 kb sequence correlated with the receptor concentration determined by¹²⁵I-T₃ displacement analysis.

Original language	English (US)
Pages (from-to)	992-998
Number of pages	7
Journal	Molecular Endocrinology
Volume	2
Issue number	10
DOIs	https://doi.org/10.1210/mend-2-10-992
State	Published - Oct 1988

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Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe. / Angel, Santos; Freake, Hedley C.; Rosenberg, Mark E. et al.
In: Molecular Endocrinology, Vol. 2, No. 10, 10.1988, p. 992-998.

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Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe

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