TY - JOUR
T1 - Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria
AU - Cusick, Sean M.
AU - O'Sullivan, Daniel J.
PY - 2000/5
Y1 - 2000/5
N2 - Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.
AB - Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.
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U2 - 10.1128/AEM.66.5.2227-2231.2000
DO - 10.1128/AEM.66.5.2227-2231.2000
M3 - Article
C2 - 10788406
AN - SCOPUS:0034057513
SN - 0099-2240
VL - 66
SP - 2227
EP - 2231
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 5
ER -