Variations in Fusion Pore Formation in Cholesterol-Treated Platelets

Solaire A. Finkenstaedt-Quinn, Sarah M. Gruba, Christy L. Haynes

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Exocytosis is a highly regulated intercellular communication process involving various membrane proteins, lipids, and cytoskeleton restructuring. These components help control granule fusion with the cell membrane, creating a pore through which granular contents are released into the extracellular environment. Platelets are an ideal model system for studying exocytosis due to their lack of a nucleus, resulting in decreased membrane regulation in response to cellular changes. In addition, platelets contain fewer granules than most other exocytosing cells, allowing straightforward measurement of individual granule release with carbon-fiber microelectrode amperometry. This technique monitors the concentration of serotonin, an electroactive molecule found in the dense-body granules of platelets, released as a function of time, with 50 μs time resolution, revealing biophysical characteristics of the fundamental exocytotic process. Variations in fusion pore formation and closure cause deviations from the classic current versus time spike profile and may influence diffusion of serotonin molecules from the site of granule fusion. Physiologically, the delivery of smaller packets of chemical messengers or the prolonged delivery of chemical messengers may represent how cells/organisms tune biological response. The goals of this work are twofold: 1) to categorize secretion features that deviate from the traditional mode of secretion and 2) to examine how changing the cholesterol composition of the platelet membrane results in changes in the pore formation process. Results herein indicate that the expected traditional mode of release is actually in the minority of granule content release events. In addition, results indicate that as the cholesterol content of the plasma membrane is increased, pore opening is less continuous.

Original languageEnglish (US)
Pages (from-to)922-929
Number of pages8
JournalBiophysical journal
Volume110
Issue number4
DOIs
StatePublished - Feb 23 2016

Bibliographical note

Funding Information:
The authors also acknowledge the National Institutes of Health (NIH) New Innovator award (DP2 OD004258-01) and the University of Minnesota for funding, as well as the National Institute of General Medical Science (NIGMS) Biotechnology Training Program Fellowship (5T32GM008347-23) for support to S.M.G.

Publisher Copyright:
© 2016 Biophysical Society.

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