Weight gain in mice on a high caloric diet and chronically treated with omeprazole depends on sex and genetic background

Milena Saqui-Salces, Amy C. Tsao, Merritt G. Gillilland, Juanita L. Merchant

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The impact of omeprazole (OM), a widely used over-the-counter proton pump inhibitor, on weight gain has not been extensively explored. We examined what factors, e.g., diet composition, microbiota, genetic strain, and sex, might affect weight gain in mice fed a high caloric diet while on OM. Inbred C57BL/6J strain, a 50: 50 hybrid (B6SJLF1/J) strain, and mice on a highly mixed genetic background were fed four diets: standard chow (STD, 6% fat), STD with 200 ppm OM (STD + O), a high-energy chow (HiE, 11% fat), and HiE chow with OM (HiE + O) for 17 wk. Metabolic analysis, body composition, and fecal microbiota composition were analyzed in C57BL/6J mice. Oral glucose tolerance tests were performed using mice on the mixed background. After 8 wk, female and male C57BL/6J mice on the HiE diets ate less, whereas males on the HiE diets compared with the STD diets gained weight. All diet treatments reduced energy expenditure in females but in males only those on the HiE + O diet. Gut microbiota composition differed in the C57BL/6J females but not the males. Hybrid B6SJLF1/J mice showed similar weight gain on all test diets. In contrast, mixed strain male mice fed a HiE + O diet gained ~40% more weight than females on the same diet. In addition to increased weight gain, mixed genetic mice on the HiE + O diet cleared glucose normally but secreted more insulin. We concluded that sex and genetic background define weight gain and metabolic responses of mice on high caloric diets and OM.

Original languageEnglish (US)
Pages (from-to)G15-G23
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume312
Issue number1
DOIs
StatePublished - 2017

Bibliographical note

Funding Information:
This study was supported by Michigan Gastrointestinal Peptide Research Center NIDDK 2P30 DK034933 (M. Saqui-Salces), NIH/NIDDK P01 DK062041 (J. Merchant), P30 DK097153 (UMICH Metabolomics Core), and 5P30 DK034933-Microbiome Core.

Publisher Copyright:
© 2017 the American Physiological Society.

Keywords

  • Acid suppression
  • Energy expenditure
  • Gastrin
  • Metabolism
  • Microbiota

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