TY - JOUR
T1 - Development of test systems for the detection of compounds that prevent the genotoxic effects of heterocyclic aromatic amines
T2 - Preliminary results with constituents of cruciferous vegetables and other dietary constituents
AU - Schwab, Christina
AU - Kassie, Fekadu
AU - Qin, Hong Min
AU - Sanyal, Ratna
AU - Uhl, Maria
AU - Hietsch, Gerhard
AU - Rabot, Sylvie
AU - Darroudi, Firouz
AU - Knasmüller, Siegfried
PY - 1999
Y1 - 1999
N2 - Over the past decades, strong efforts have been made to identify dietary constituents that protect against the genotoxic effects of heterocyclic aromatic amines (HAAs). However, most of the methods that have been used, in particular in vitro assays that require the addition of exogenous enzyme homogenates, have only a limited predictive value because important protective mechanisms are not adequately represented and may give misleading results. Therefore, we attempted to develop improved test systems, namely assays, with human hepatoma cells and single-cell gel electrophoresis (SCGE) tests with rats. Genotoxicity tests with human derived Hep G2 cells reflect the genotoxic effects of HAAs better than other in vitro systems. They also enable the detection of protective effects since the human derived hepatoma cells possess phase I and phase II enzymes that are involved in the activation/detoxification of the amines. The most appropriate endpoint for experiments with Hep G2 cells appears to be micronucleus induction, but protocols for other endpoints are available as well. The second promising model is the SCGE ('comet') assay with rats that was used successfully to measure protective effects of constituents of cruciferous vegetables against 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in the liver and in the colon mucosa. The present study describes the experimental design of the new approaches, as well as results obtained with various dietary constituents.
AB - Over the past decades, strong efforts have been made to identify dietary constituents that protect against the genotoxic effects of heterocyclic aromatic amines (HAAs). However, most of the methods that have been used, in particular in vitro assays that require the addition of exogenous enzyme homogenates, have only a limited predictive value because important protective mechanisms are not adequately represented and may give misleading results. Therefore, we attempted to develop improved test systems, namely assays, with human hepatoma cells and single-cell gel electrophoresis (SCGE) tests with rats. Genotoxicity tests with human derived Hep G2 cells reflect the genotoxic effects of HAAs better than other in vitro systems. They also enable the detection of protective effects since the human derived hepatoma cells possess phase I and phase II enzymes that are involved in the activation/detoxification of the amines. The most appropriate endpoint for experiments with Hep G2 cells appears to be micronucleus induction, but protocols for other endpoints are available as well. The second promising model is the SCGE ('comet') assay with rats that was used successfully to measure protective effects of constituents of cruciferous vegetables against 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in the liver and in the colon mucosa. The present study describes the experimental design of the new approaches, as well as results obtained with various dietary constituents.
KW - Antigenotoxicity
KW - Antimutagens
KW - Chemoprotection
KW - Genotoxicity
KW - Hep G2 cells
KW - Heterocyclic aromatic amines
UR - http://www.scopus.com/inward/record.url?scp=0033003415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033003415&partnerID=8YFLogxK
M3 - Review article
C2 - 15281222
AN - SCOPUS:0033003415
SN - 0731-8898
VL - 18
SP - 109
EP - 118
JO - Journal of Environmental Pathology, Toxicology and Oncology
JF - Journal of Environmental Pathology, Toxicology and Oncology
IS - 2
ER -